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efficient production of antifungal proteins in plants

efficient production of antifungal proteins in plants

Efficient Production of Antifungal Proteins in Plants ...

Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems.

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Efficient production of antifungal proteins in plants ...

Nov 22, 2018  Production of the antifungal protein AfpB in leaf apoplasts of N. benthamiana plants AfpB is an AFP whose gene was identified in the genome of the postharvest phytopathogenic fungus P. digitatum but that, unlike other AFPs, is not naturally produced by the fungus (Garrigues et al ., 2016 ).

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Efficient production of antifungal proteins in plants ...

Dec 06, 2018  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus Xiaoqing Shi , 1 Teresa Cordero , 2 Sandra Garrigues , 3 Jose F. Marcos , 3 José‐Antonio Daròs , 2 and María Coca 1

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Addgene: Efficient production of antifungal proteins in ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotech Journal (2018) Article

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Efficient production of antifungal proteins in plants ...

Jun 01, 2019  This work was supported by grants BIO2017-83184-R, BIO2015-68790-C2-1-R and BIO2015-68790-C2-2-R and through the 'Severo Ochoa Programme for Centres of Excellence in RD' (SEV-2015-0533) from Spanish Ministerio de Ciencia, Innovacion y Universidades (co-financed FEDER funds) and by the CERCA Programme/Generalitat de Catalunya. We thank Laia Castillo for technical

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Efficient production of antifungal proteins in plants ...

Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems.

get price

Addgene: Efficient production of antifungal proteins in ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotech Journal (2018) Article

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Efficient production of antifungal proteins in plants ...

This work was supported by grants BIO2017-83184-R, BIO2015-68790-C2-1-R and BIO2015-68790-C2-2-R and through the 'Severo Ochoa Programme for Centres of Excellence in RD' (SEV-2015-0533) from Spanish Ministerio de Ciencia, Innovacion y Universidades (co-financed FEDER funds) and by the CERCA Programme/Generalitat de Catalunya. We thank Laia Castillo for technical support.

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Plants as efficient antifungal factories - Phys

Dec 11, 2018  More information: Xiaoqing Shi et al. Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus, Plant

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Plants as antifungal factories -- ScienceDaily

Dec 10, 2018  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal ,

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Plants produce antifungal proteins Global Plant ...

Dec 18, 2018  Phys.Org Plants as efficient antifungal factories December 11, 2018, Asociacion RUVID Nicotiana plants. Credit: Asociación RUVIDResearchers report that they can efficiently produce antifungal proteins in plants based on a modified tobacco mosaic virus. The results of this research, which could have a major impact in the agri-food industry, have been published in the Plant

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Plants as antifungal factories CRAG Centre for ...

Researchers from the Spanish Research Council (CSIC) at the Centre for Research in Agricultural Genomics (CRAG) and the Institute for Plant Molecular and Cellular Biology (IBMCP), in collaboration with the IATA, have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in plants.The results of this research, that could impact the agri-food and ...

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Efficient production and characterization of the novel and ...

1 Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum Sandra Garrigues1, Mónica Gandía1, Crina Popa2, Attila Borics3, Florentine Marx4, María Coca2, Jose F. Marcos1, Paloma Manzanares1* 1Department of Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones ...

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Plant antifungal proteins and their applications in ...

May 15, 2015  Plants have developed various mechanisms to defend themselves against these fungi which include the production of low-molecular-weight secondary metabolites and proteins and peptides with antifungal activity. In this review, families of plant antifungal proteins (AFPs) including defensins, lectins, and several others will be summarized.

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Production of a defensin-like antifungal protein NFAP from ...

Nov 19, 2013  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotechnol J, 17(6):1069-1080, 06 Dec 2018 Cited

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Biotechnological Production of the Cell Penetrating ...

This study reports the efficient production of PAF102 in P. pastoris when fused to the rice plant Oleosin 18, whereas no accumulation of Cecropin A was detected. The Ole18-PAF102 fusion protein targets the lipid droplets of the heterologous system where it accumulates to high levels. Interestingly, the production of this fusion protein induces ...

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Use of a PTGS-MAR expression system for efficient in ...

Use of a PTGS-MAR expression system for efficient in planta production of bioactive Arabidopsis thaliana plant defensins. Sels J(1), Delauré SL, Aerts AM, Proost P, Cammue BP, De Bolle MF. Author information: (1)Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Heverlee, Belgium.

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Plants as antifungal factories EurekAlert!

Dec 10, 2018  Researchers from three research institutes in Spain have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in the leaves of the plant

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trichoderma as biocontrol agent

The offered volume intends to review the biological control theme of phytonematodes from several prospects: ecological; applicative as well as commercial state of the art; understanding the mode-of-action of various biocontrol systems; ... more and more biological control agents. Increase Dry matter Production substantially. Ascospores are hyaline or green and typically spinulose. Trichoderma ...

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Plants produce antifungal proteins Global Plant ...

Dec 18, 2018  Phys.Org Plants as efficient antifungal factories December 11, 2018, Asociacion RUVID Nicotiana plants. Credit: Asociación RUVIDResearchers report that they can efficiently produce antifungal proteins in plants based on a modified tobacco mosaic virus. The results of this research, which could have a major impact in the agri-food industry, have been published in the Plant

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New antifungal proteins in plant biofactories

May 31, 2019  New antifungal proteins in plant biofactories. Shi X, Cordero T, Garrigues S, et al. 2018.Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus.Plant Biotechnology Journal, DOI: 10.1111/pbi.13038

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A Review on Antifungal Efficiency of Plant Extracts ...

Jun 15, 2021  Fluconazole is an antifungal drug with low molecular weight and high solubility in water that does not require plasma protein to travel to the plasma, which it reaches within 1–2 h after oral consumption. Fluconazole’s efficiency in treating disease is the same as griseofulvin.

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Efficient production and characterization of the novel and ...

Nov 07, 2017  Garrigues, S., Gandía, M., Popa, C. et al. Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum. Sci Rep 7, 14663 (2017 ...

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Plants as antifungal factories EurekAlert!

Dec 10, 2018  Researchers from three research institutes in Spain have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in the leaves of the plant

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Efficient production and characterization of the novel and ...

1 Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum Sandra Garrigues1, Mónica Gandía1, Crina Popa2, Attila Borics3, Florentine Marx4, María Coca2, Jose F. Marcos1, Paloma Manzanares1* 1Department of Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones ...

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Plants as Anti-fungal Factories - Infection Control Today

Dec 11, 2018  Coca's research group, in collaboration with the IATA's researcher Jose F. Marcos, aims to develop new antifungal compounds based on the antifungal proteins (AFPs) secreted by filamentous fungi. The problem is that the synthesis of these compounds is extremely complex; hence their exploitation requires efficient, sustainable and safe production ...

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Antifungal Properties of Bioactive Compounds from Plants

Antifungal Properties of Bioa ctive Compounds from Plants 87 2.4 Nitrogenous compounds A large variety of plant secondary metabolites ha ve nitrogen in their structure. Included in this category are such well-known anti-defenses as alkaloids, amines, cyanogenic glycosides, non-protein amino acids, glucosinolates, alkamides and peptides (Wink

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Antifungal Protein - an overview ScienceDirect Topics

Antifungal proteins are a heterogeneous assembly of structurally diverse proteins produced by a wide range of organisms encompassing mammals, insects, flowering plants, gymnosperms, fungi, and bacteria. From: Handbook of Biologically Active Peptides, 2006. Download as PDF. About this page.

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Plant immune responses to pathogen infection ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. (2019) Plant Biotechnology Journal (published on line doi: 10.1111/pbi13038). R Salvador-Guirao, P Baldrich, S Tomiyama, Y-I Hsing, K

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Therapeutic recombinant protein production in plants ...

Nov 28, 2019  Therapeutic protein production in plants is an area of great potential for increasing and improving the production of proteins for the treatment or prevention of disease in humans and other animals. There are a number of key benefits of this technique for scientists and society, as well as regulatory challenges that need to be overcome by ...

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Production of an antifungal protein for control of ...

May 01, 2004  Abstract. A plant pathogenic fungus, Colletotrichum lagenarium, causing watermelon anthracnose, was isolated from naturally infected leaves, stems, and fruits of watermelon.A bacterial strain, MET0908, showing a potent antifungal activity against C. lagenarium, was isolated from soil.An antifungal protein was purified by 30% ammonium sulfate saturation and concentrated using

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New viral vector for efficient production of target ...

A new potato virus X (PVX)-based viral vector for superproduction of target proteins in plants has been constructed. The triple gene block and coat protein gene of PVX were substituted by green fluorescent protein. This reduced viral vector was delivered into plant cells by agroinjection (injection of Agrobacterium tumefaciens cells, carrying viral vector cDNA within T-DNA, into plant leaves ...

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Bioactive Natural Products in Plant Disease Control ...

Jan 01, 2018  The extracted algae is less efficient for biofuel production, however, because laminarin may constitute up to 35% of the total dry weight and a larger portion of the carbohydrate is needed for biofuel production . Biofuel yield from plant sources is reduced by phenolic-rich, nonstructural extractives that hinder enzyme activity and ...

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